Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18
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چکیده
منابع مشابه
Adherence patterns of diarrheagenic Escherichia coli strains isolated from children with gastrointestinal diarrhea and matched controls in a Hep-2 cell adhesion assay
In the present study, carbon species including organic carbon (OC), elemental carbon (EC), and water-soluble organic carbon (WSOC) concentration in PM2.5 were assessed at an urban site of Tehran, Iran during March to June 2014. The PM2.5 samples were collected using an frmOMNITM Ambient Air Sampler. Thermal gravimetric analysis (TGA) was used to analyze OC and EC. The results showed that PM2.5 ...
متن کاملThe hydrophobic surface protein layer of enteroaggregative Escherichia coli strains.
The surface of three strains of enteroaggregative Escherichia coli (EAggEC) and three strains of enteropathogenic E. coli (EPEC) were examined using the freeze-substitution technique of electron microscopy and as a result an electron dense surface layer was found only on EAggEC strains but not on EPEC strains. The analysis of the outer membrane proteins by polyacrylamide gel electrophoresis rev...
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Enteroaggregative Escherichia coli (EAEC) is associated with diarrhea in Spanish travelers to developing countries. In this study, the polymerase chain reaction was used to test EAEC isolates for genes encoding putative virulence factors, including EAEC adhesins, the plasmid-encoded toxin (Pet), a heat-stable enterotoxin (EAST), and Shigella enterotoxins 1 and 2 (ShET1 and ShET2). Findings incl...
متن کاملDetection of enteroaggregative Escherichia coli with formalin-preserved HEp-2 cells.
Formalin-stored HEp-2 cells were used to assay Escherichia coli for adherence. Cells refrigerated in formalin for up to 28 days and used in a wet assay format demonstrated an assay sensitivity ranging from 94 to 98% to detect enteroaggregative E. coli (EAEC). HEp-2 cells first fixed and stored with formalin and then stored dry in ambient conditions for 6 weeks demonstrated an assay sensitivity ...
متن کاملadherence patterns of diarrheagenic escherichia coli strains isolated from children with gastrointestinal diarrhea and matched controls in a hep-2 cell adhesion assay
in the present study, carbon species including organic carbon (oc), elemental carbon (ec), and water-soluble organic carbon (wsoc) concentration in pm2.5 were assessed at an urban site of tehran, iran during march to june 2014. the pm2.5 samples were collected using an frmomnitm ambient air sampler. thermal gravimetric analysis (tga) was used to analyze oc and ec. the results showed that pm2.5 ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1997
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(97)00011-6